ACELL Mar. 45/3
نویسندگان
چکیده
Koyama, Yu, Tadashi Yamamoto, Tatsuo Tani, Kouei Nihei, Daisuke Kondo, Haruko Funaki, Eishin Yaoita, Katsutoshi Kawasaki, Nobuaki Sato, Katsuyoshi Hatakeyama, and Itaru Kihara. Expression and localization of aquaporins in rat gastrointestinal tract. Am. J. Physiol. 276 (Cell Physiol. 45): C621–C627, 1999.—A family of water-selective channels, aquaporins (AQP), has been demonstrated in various organs and tissues. However, the localization and expression of the AQP family members in the gastrointestinal tract have not been entirely elucidated. This study aimed to demonstrate the expression and distribution of several types of the AQP family and to speculate on their role in water transport in the rat gastrointestinal tract. By RNase protection assay, expression of AQP1–5 and AQP8 was examined in various portions through the gastrointestinal tract. AQP1 and AQP3 mRNAs were diffusely expressed from esophagus to colon, and their expression was relatively intense in the small intestine and colon. In contrast, AQP4 mRNA was selectively expressed in the stomach and small intestine and AQP8 mRNA in the jejunum and colon. Immunohistochemistry and in situ hybridization demonstrated cellular localization of these AQP in these portions. AQP1 was localized on endothelial cells of lymphatic vessels in the submucosa and lamina propria throughout the gastrointestinal tract. AQP3 was detected on the circumferential plasma membranes of stratified squamous epithelial cells in the esophagus and basolateral membranes of cardiac gland epithelia in the lower stomach and of surface columnar epithelia in the colon. However, AQP3 was not apparently detected in the small intestine. AQP4 was present on the basolateral membrane of the parietal cells in the lower stomach and selectively in the basolateral membranes of deep intestinal gland cells in the small intestine. AQP8 mRNA expression was demonstrated in the absorptive columnar epithelial cells of the jejunum and colon by in situ hybridization. These findings may indicate that water crosses the epithelial layer through these water channels, suggesting a possible role of the transcellular route for water intake or outlet in the gastrointestinal tract.
منابع مشابه
ACELL Mar. 45/3
MARK O. BEVENSEE,1 ESTHER BASHI,1 WOLF-RÜDIGER SCHLUE,2 GREGORY BOYARSKY,3 AND WALTER F. BORON1 1Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520; 2Institut für Neurobiologie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; and 3Department of Physiology and Biophysics, University of Texas Medical Branch, Galvesto...
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AKI YAMADA,1 SUSUMU OHYA,2 MASARU HIRANO,2 MINORU WATANABE,2 MICHAEL P. WALSH,3 AND YUJI IMAIZUMI1 1Department of Pharmacology and Therapeutics and 2Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan; and 3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Ca...
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Farge, Emmanuel, David M. Ojcius, Agathe Subtil, and Alice Dautry-Varsat. Enhancement of endocytosis due to aminophospholipid transport across the plasma membrane of living cells. Am. J. Physiol. 276 (Cell Physiol. 45): C725– C733, 1999.—Formation of intracellular vesicles is initiated by membrane budding. Here we test the hypothesis that the plasma membrane surface area asymmetry could be a dr...
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Kleine, Teri J., Gerald J. Gleich, and Simon A. Lewis. Eosinophil peroxidase increases membrane permeability in mammalian urinary bladder epithelium. Am. J. Physiol. 276 (Cell Physiol. 45): C638–C647, 1999.—Eosinophil peroxidase (EPO), a cationic protein found in eosinophils, has been reported to be cytotoxic independent of its peroxidase activity. This study investigated with electrophysiologi...
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Amemiya, Morimasa, Kaoru Tabei, Eiji Kusano, Yasushi Asano, and Robert J. Alpern. Incubation of OKP cells in low-K1 media increases NHE3 activity after early decrease in intracellular pH. Am. J. Physiol. 276 (Cell Physiol. 45): C711–C716, 1999.—Chronic hypokalemia increases the activity of proximal tubule apical membrane Na1/H1 antiporter NHE3. The present study examined the effect of the incub...
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Maréchal, Hervé, Hélene Jammes, Bernard Rossignol, and Philippe Mauduit. EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland. Am. J. Physiol. 276 (Cell Physiol. 45): C734– C746, 1999.—This study was designed to demonstrate the presence of epidermal growth factor (EGF) in the rat exorbital lacrimal gland. EGF precursor gene transcription was demonstra...
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